Activity

The MS4Omics platform of the PRISM Inserm U1192 laboratory offers expertise in proteomics and mass spectrometry applied to different fields.

In proteomics, our focus lies in non-targeted methodologies, including bottom-up, shotgun, and top-down approaches, as well as interactomics techniques such as cross-linking MS and BioID. We have particular expertise in tissue analysis through spatial proteomics, FFPE tissue analysis, and proteogenomics. Additionally, our capabilities extend to the purification, quantification, and analysis of extracellular vesicles. The link between the platform and the laboratory enables us to offer unique expertise.

MS4Omics takes on requests from local, regional and national academics and industry, or from outside France.

List of skills

  • Large-scale proteomic analysis (bottom-up, shotgun, top-down) label-free quantification and analysis of large cohorts (>100 samples)
  • Sample preparation for large-scale proteomics (FASP, iST, SP3) irrespective of sample storage mode (fresh-frozen, fixed, FFPE) and for any biological material (fluids, organs, biopsies, tissue sections, organoids, spheroids, extracellular vesicles)
  • Proteogenomics and alternative proteome (AltProt and small protein)
  • Tissue imaging and profiling by mass spectrometry (MALDI, DESI, SpiderMass)
  • Sample preparation for MS imaging (cutting, dewaxing, washing, antigen unmasking, enzymatic digestion) for fresh-frozen, fixed and FFPE tissues, and for all biological tissue material (organs, biopsies, tissue sections, organoids, spheroids, plants).
  • Spatial proteomics analysis of tissue sections including FFPE
  • Interactomics analysis by cross-linking MS and BioID
  • Analysis of proteomics and MS imaging data with multivariate statistical analysis and deep-learning tools
  • Metabolomics, lipidomics & glycoproteomics
  • Spatially-Resolved Omics
  • Extracellular Vesicles (including exosomes) purification and analysis

At 3D Cell Omics, we offer comprehensive support at every stage of your project, from the cultivation of cell lines, creation of 3D models to data analysis, and ongoing maintenance of cell cultures.

We provide access to cutting-edge technologies, including the creation of mixed spheroids/organoids, advanced invasion computation software, 3D organ bioprinting capabilities, and precise isolation and quantification of exosomes. Moreover, our expertise extends to data processing and statistical analysis, enabling accurate identification and relative quantification of markers crucial to your research objectives.

List of skills

  • Large index of cell lines (>100 different cell lines from different species and types).
  • Mixed spheroid / tumor organoids creation: association of cancer cells & immune cells in 3D culture to mimic tissue microenvironment.
  • Large scale of different test: invasion test, viability test, MS/MS, imaging, fluorescence.
  • 3D cell bioprinting.
  • Exosome isolation and count with Nanosight (from cell secretomes and body fluids).
  • Culture from isolated organs, biopsies, tissue sections, isolated cells layer, 2D or 3D cell culture…
  • Combined multi-omics analysis (metabolomics, lipidomics and proteomics).
  • Design, execution and analysis of experiments.